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bac contig  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bac contig
    Bac Contig, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bac+contig/Bacitracin/pm27392293-47-14-38
    Average 99 stars, based on 1 article reviews
    bac contig - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Genomic Sequencing:

    Article Title: A spontaneous mutation in MutL-Homolog 3 (HvMLH3) affects synapsis and crossover resolution in the barley desynaptic mutant des10.
    Article Snippet: .. Primers were designed to amplify the genomic sequences of the six genes within the BAC contig and all other possible syntenic genes and the PCR products sequenced using the big dye v.3.1 reaction kit and analysed on an ABI Prism 3730 (Applied Biosystems, Foster City, CA, USA). .. For cDNA sequencing, mRNA from young inflorescences and anthers from BW230 (des10) and Bowman was extracted using an RNA extraction kit New Phytologist (2016) 2016 The Authors New Phytologist 2016 New Phytologist Trustwww.newphytologist.com Research New Phytologist2 (Qiagen) in the presence of DNAseI. cDNA was made using the standard protocol of the Superscript III kit (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) and sequenced using specific primers encompassing the deleted region.

    BAC Assay:

    Article Title: A spontaneous mutation in MutL-Homolog 3 (HvMLH3) affects synapsis and crossover resolution in the barley desynaptic mutant des10.
    Article Snippet: .. Primers were designed to amplify the genomic sequences of the six genes within the BAC contig and all other possible syntenic genes and the PCR products sequenced using the big dye v.3.1 reaction kit and analysed on an ABI Prism 3730 (Applied Biosystems, Foster City, CA, USA). .. For cDNA sequencing, mRNA from young inflorescences and anthers from BW230 (des10) and Bowman was extracted using an RNA extraction kit New Phytologist (2016) 2016 The Authors New Phytologist 2016 New Phytologist Trustwww.newphytologist.com Research New Phytologist2 (Qiagen) in the presence of DNAseI. cDNA was made using the standard protocol of the Superscript III kit (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) and sequenced using specific primers encompassing the deleted region.

    Article Title: Growth and function of the embryonic heart depend upon the cardiac-specific L-type calcium channel alpha1 subunit.
    Article Snippet: .. The BAC contig was assembled using the proper (Molecular Probes) were mixed in 0.2 M KCl and pressure injected into wild-type donor embryos (AB) at the 1- to 2-cell stage. ..

    Polymerase Chain Reaction:

    Article Title: A spontaneous mutation in MutL-Homolog 3 (HvMLH3) affects synapsis and crossover resolution in the barley desynaptic mutant des10.
    Article Snippet: .. Primers were designed to amplify the genomic sequences of the six genes within the BAC contig and all other possible syntenic genes and the PCR products sequenced using the big dye v.3.1 reaction kit and analysed on an ABI Prism 3730 (Applied Biosystems, Foster City, CA, USA). .. For cDNA sequencing, mRNA from young inflorescences and anthers from BW230 (des10) and Bowman was extracted using an RNA extraction kit New Phytologist (2016) 2016 The Authors New Phytologist 2016 New Phytologist Trustwww.newphytologist.com Research New Phytologist2 (Qiagen) in the presence of DNAseI. cDNA was made using the standard protocol of the Superscript III kit (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) and sequenced using specific primers encompassing the deleted region.

    Injection:

    Article Title: Growth and function of the embryonic heart depend upon the cardiac-specific L-type calcium channel alpha1 subunit.
    Article Snippet: .. The BAC contig was assembled using the proper (Molecular Probes) were mixed in 0.2 M KCl and pressure injected into wild-type donor embryos (AB) at the 1- to 2-cell stage. ..



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